Figure 7.
Figure 7. Generation of CD45RAhiLin-CD10+ from UCB and BM CD34+CD45RAhiLin- cells. CD34+CD45RAhiLin- cells were sorted by FACS from UCB or BM CD34+ HPCs and cultured for 4 to 5 days onto MS5 cells in the presence of FL and TSLP. The recovered cells, gated based on CD34 expression (not shown), were then analyzed by FACS. Data are from 1 of 3 experiments.

Generation of CD45RAhiLin-CD10+ from UCB and BM CD34+CD45RAhiLin- cells. CD34+CD45RAhiLin- cells were sorted by FACS from UCB or BM CD34+ HPCs and cultured for 4 to 5 days onto MS5 cells in the presence of FL and TSLP. The recovered cells, gated based on CD34 expression (not shown), were then analyzed by FACS. Data are from 1 of 3 experiments.

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