Expression of the hematopoietic markers on the rescued cells from AML1-deficient P-Sp culture. Flow cytometric profiles of the cells stained with antibodies against Gr1/Mac1, CD45, CD34, Sca1, and c-Kit. Note that the rescued cells from AML1-deficient P-Sp regions are GFP-positive and express various hematopoietic cell surface markers. Flow cytometric profiles of the rescued cells are similar to those of hematopoietic cells in wild-type P-Sp culture. GFP intensity (marking retrovirally transduced cells) is plotted on the x-axis and intensity of counterstaining of hematopoietic surface markers is plotted on the y-axis. Isotype-matched control staining of the hematopoietic cells from wild-type P-Sp regions is also shown.