Figure 1.
Group O and A1 RBCs can be clearly distinguished by Dolichos biflorus–FITC staining and flow cytometry. A sample of washed group A1 blood from patient 1 (A) was mixed with washed group O blood from donor 1 (C) in a ratio of 1:3 (patient 1 and donor 1 blood mixture is seen in panel E). A sample of patient 2 group A1 blood (B) was mixed with donor 2 group O blood (D) in a 1:1 ratio (mixture of patient group A1 blood and donor 2 group O blood is seen in panel F). All were stained with Dolichos biflorus–FITC and analyzed by flow cytometry. Above are scattergrams (E-F) showing that samples of the 2 blood groups can be clearly discriminated by flow cytometry.
