Transfusion of human RBC microvesicles to mice results in transfer of CD59. Six NOD/SCID mice were administered transfusions of a mixture of microvesicles derived from outdated units of human blood. An additional 2 received RBCs. CD59 mAbs that did not cross-react with mouse cells was used to stain TER119 and CD24⁺ mouse erythrocytes. Data from 7 representative mice are seen above (A). When double-positive–stained (TER119-PE/CD59-FITC) mouse cells were sorted and looked at using a fluorescent light microscope (× 60) mouse RBCs showed homogeneous membrane staining under the FITC filter, suggesting uniform transfer (B).