Immunofluorescence microscopy analysis of MRP4 localization in human platelets. Platelets were adhered on collagen, fixed, permeabilized, and incubated with the primary anti-MRP4 antibodies SNG (A, C) and MLP (E) or, as control, with the respective preimmune sera (B, F). (D) Staining with SNG after preincubation with the synthetic peptide used to generate the SNG antiserum. Images were obtained by confocal laser scanning microscopy using an Alexa488-conjugated antirabbit secondary antibody and identical parameters for each set.