Figure 5.
![Figure 5. Transport of cyclic nucleotides in platelet membranes. Platelet membrane vesicles (100 μg protein) were incubated with [3H]cGMP (left panel) or [3H]cAMP (right panel) (4 μM) in the presence of 4 mM ATP (▴) or 4 mM 5′-AMP (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3). The rate of net ATP-dependent transport (▪) was calculated by subtracting transport in the presence of 5′-AMP as a blank from transport in the presence of ATP. Right panel: ATP-dependent transport of cAMP in the presence (♦) or absence (▪) of 10 μM dipyridamole.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/12/10.1182_blood-2003-12-4330/5/zh80230470130005.jpeg?Expires=1735830495&Signature=Ms76AVGh6~pEEVKtI75Kd6gmhIoRR4Hd33Q~CGmINP9WXqi5HJm61F8VSjpaTkoi3dz-7d4UOteCS2syZLAtcsrn63LHhDlp4vpSSScVv-tN8iMGp2VSHGkutLZ4BXQLTyAqYBcyf-OEOY2ZXJ48cTjcCi6EWdr~JIZ~FExZUDqlUKqfFDw1b9ccLasUIOE5O2r73QFwpnIrObiQ2RtThpMeMI3D9fFXmvDtKRtvWE2NiplSToWBcCdTciWlMNdWrUgtCx~TycUI07S7OznfgHpLq7LRregcAORMNYXiifYTXjZ0fBz9TTZMhYxR2KZgKKRizl6o~wAXr760KmL5vA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transport of cyclic nucleotides in platelet membranes. Platelet membrane vesicles (100 μg protein) were incubated with [3H]cGMP (left panel) or [3H]cAMP (right panel) (4 μM) in the presence of 4 mM ATP (▴) or 4 mM 5′-AMP (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3). The rate of net ATP-dependent transport (▪) was calculated by subtracting transport in the presence of 5′-AMP as a blank from transport in the presence of ATP. Right panel: ATP-dependent transport of cAMP in the presence (♦) or absence (▪) of 10 μM dipyridamole.
