Figure 7.
![Figure 7. Transport of ADP in platelet membranes. (A-D) Platelet membrane vesicles (100 μg protein; crude membranes) were incubated with [3H]ADP (1 μM) in the presence of 0.4 mM ATP (▪) or 0.4 mM ATP + 1 mM orthovanadate (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3; 1 pmol × mg protein–1 = 1724 DPM). (A-B) Vesicles were preincubated for 45 minutes at 4°C in standard incubation buffer containing 250 mM sucrose (A) or in buffer containing 1 M sucrose (B). (C-D) ADP transport in the absence (C) or presence (D) of 100 μM dipyridamole.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/12/10.1182_blood-2003-12-4330/5/zh80230470130007.jpeg?Expires=1735508293&Signature=EXyDKYBpD~j-wwyM1PAf3tAh6zjV5UyM1Lu6MuhQ1ay77TF4tBo-L9d8OGyiuOMJpzkM2UUTOG5XaAJWwPfZduwhGNdrCp9EU3KjTCUbevfxPGNm4pZhiQQgJE8bnL03kk3ir99dccgdxLNkX7WwrAAlntcBLV09JTot4IOO4EbB0~kxKudfTeyd3XlOBRt5pPoFGBF1E2LgEMp60edtNTp~mKiHkMcqmcAVy0HNAVYy5nkcmWaA1rZnt-H7diW3jsFucHHTI5wx9CJXYDzEZ7~e4aIcHI3lJR5qlyUxSc05jIc4lh9YSpNPH8vpudbBfGwnKlnREQJYKvotAp8qKg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Transport of ADP in platelet membranes. (A-D) Platelet membrane vesicles (100 μg protein; crude membranes) were incubated with [3H]ADP (1 μM) in the presence of 0.4 mM ATP (▪) or 0.4 mM ATP + 1 mM orthovanadate (♦), and the vesicle-associated radioactivity was determined as described in “Patients, materials, and methods” (mean values ± SD, n = 3; 1 pmol × mg protein–1 = 1724 DPM). (A-B) Vesicles were preincubated for 45 minutes at 4°C in standard incubation buffer containing 250 mM sucrose (A) or in buffer containing 1 M sucrose (B). (C-D) ADP transport in the absence (C) or presence (D) of 100 μM dipyridamole.
