Similar self-renewal capacity seen for AML1+/– and +/+ LTR-HSCs. (A) 1 × 10⁶ BM cells (Ly5.2⁺) from AML1+/+ or +/– mice were injected into lethally irradiated recipients. Three to four months after transplantation, BM cells were harvested from animals that had received transplants, donor-derived Ly5.2⁺ cells were sorted by FACS, and 0.5 to 1 × 10⁶ Ly5.2⁺ cells were injected into the secondary recipients. This procedure was then repeated sequentially 3 to 4 months after each transplantation. The percentage of mice surviving from 4 sequential cycles is shown (n = 9-10 mice/group). (B) BM cells from secondary recipients were analyzed for multilineage engraftment by FACS. The percentage of Ly5.2⁺ T cells (CD3+), B cells (B220+), erythroid (Ter119+), and GR1+ and Mac1+ myeloid cells is shown. (n = 4 mice/group). Error bars represent standard deviation.