Dectin-1-mediated phagocytosis resembles that of FcγR in transduced NIH-3T3 fibroblasts. (A) The effect of pharmacologic inhibitors on Dectin-1-mediated zymosan uptake was determined using flow cytometry, as described in “Materials and methods.” Zymosan phagocytosis was synchronized and allowed to occur for 60 minutes before quantitation. DMSO (dimethyl sulfoxide) represents a solvent-alone control. (B) Dectin-1-expressing NIH-3T3 cells were transfected with the dominant-negative Rho-GTPase constructs N19RhoA, N17Rac-1, and N17Cdc42 24 hours prior to quantitation of phagocytic capacity by flow cytometry. A transfection control, without DNA, was included to demonstrate the effects of the transfection on particle uptake. The values shown are the mean ± SEM of data pooled from 3 independent experiments and are expressed relative to uptake in cells in medium only. ^*P < .05.