Regulatory T-cell gene expression is not increased in the FC or the T_BM populations. Real-time PCR analysis of CD8⁺/TCR^- FCs (n = 4) or CD8⁺/TCR⁺ T_BM (n = 4) isolated from donor BM compared with unstimulated control B6 splenocytes sorted for the CD4⁺CD25⁺ regulatory T-cell phenotype or the CD4⁺CD25^- or CD4^-CD25^- nontolerogenic phenotype. Individual samples were analyzed for the presence of mRNA encoding GITR, CTLA4, and FoxP3 and were semiquantitated as a mean ± SD percentage of baseline GAPDH expression.