Proliferative response of iNKT cells to IL-7. (A) CD8-depleted thymocytes, CB cells, or PBMCs were stained with CFSE and cultured for 7 days in the presence of IL-7 and anti-CD1d blocking mAb. iNKT cells were identified with PE-6B11 and plotted against CFSE fluorescence. (B) Distribution of CFSE-fluorescence in CD4⁺ versus CD4^- subsets of iNKT cells from adult PBMCs cultured with IL-7 + anti-CD1d mAb. Representative diagrams from 5 independent experiments are shown.