Figure 3.
Repopulation of thymus of RAG2-/- γc-/- mice, 11 weeks after intraperitoneal injection with CD34+ hematopoietic stem cells from fetal liver. FACS profiles of thymi. Thymocytes were stained with antibodies against stem cell markers CD34 and CD38, T-cell markers TCR αβ and TCR γδ, CD1, CD3, CD4, CD8, CD25, CD45RA, NK cell markers CD16 and CD56, plasmacytoid DC markers CD123 and BDCA2, and B-cell marker CD19. Cells in the analysis of CD16 and CD56 were gated not only on CD45+ cells but also on CD3- cells.