Figure 4.
Repopulation of BM, spleen, liver, and lung of RAG2-/- γc-/- mice 11 weeks after intraperitoneal injection with CD34+ hematopoietic stem cells from fetal liver. (A) FACS profiles of BM. BM cells were stained with antibodies against stem cell markers CD34 and CD38; T-cell markers CD3, CD4, CD25; NK cell markers CD16 and CD56; plasmacytoid DC markers CD123 and BDCA2; B cell markers CD11, CD19, CD20; IgM and IgD; and myeloid cells markers CD11c and CD14. Cells in the analysis of IgM and IgD are not only gated on CD45+ cells but also on CD19+ cells. (B) FACS profiles of spleen. Spleen cells were stained with the same antibodies as BM cells. (C) FACS profiles of liver. Liver cells were stained with antibodies against stem cell markers CD34 and CD38, T cell marker CD3, plasmacytoid DC markers CD123 and CD4, and B cell marker CD19. (D) FACS profiles of lung. Lung cells were stained with antibodies against T-cell marker CD3, plasmacytoid DC markers CD123 and CD4, and B-cell marker CD19.