Multilineage presence of GFP⁺-expressing cells in mice that underwent transplantation with lentivirus-transduced human hematopoietic progenitors. Newborn RAG2^-/- γc^-/- mice were intraperitoneally injected with human CD34⁺ hematopoietic stem cells isolated from fetal liver and transduced with the pTRIPΔU3-EF1α p53RNAi lentivirus. (A) Mononuclear cell suspensions from the thymi and spleens of these mice were stained with various human-specific monoclonal antibodies and analyzed by flow cytometry. Cells positive for human CD45 were gated and further analyzed, comparing the GFP⁺ and GFP^- populations. Examples of T-cell development in thymi and spleens are given. Cells were stained with antibodies directed to CD3, CD4, CD8, and CD56 and analyzed by FACS. (B) Vβ family representation from p53i GFP⁺-expressing T cells in mice that underwent transplantation with lentivirus-transduced human hematopoietic progenitors. RNA was isolated from T cells, and the presence of different Vβ subsets was determined using RT-PCR.