Detection of telomerase activity of the 8 TERC mutations mixed with WT TERC. Detection of telomerase activity is shown in vitro (A) and in vivo (B). The arrows denote the start of the TRAP ladder (TRAP) and the corresponding internal control (IC). For each experiment, 100 ng WT TERC RNA (A) or 4 μg WT TERC plasmid (B) was mixed with the same amount of mutant TERC, and the resulting telomerase activity was measured by TRAP assay.