Figure 1.
Human NK cells and NK cell lines express TLR3 and TRIF/TICAM-1 mRNA. Total RNA from highly purified fresh (both panels, left) and short-term cultured (A-B, middle) NK cells from different donors and a panel of NK cell lines (both panels, right) was subjected to RT-PCR with specific primers for TLR3 (A) and TRIF/TICAM-1 (B); β-actin amplification, as control, is shown in the lower lanes. A longer exposure for the TLR3 sample in NKL is shown in panel A (NKLb). The data shown are representative of 1 out of 4 independent experiments.