Figure 6.
Inhibition of PI3K activity selectively blocked LFA-1 ligation-induced production of ROSs. U937 cells (6 × 105 cells for each condition) were preincubated with LY294002 (20 μM), Akt inhibitor, and U0126 (30 μM) for 15 minutes, then labeled with H2DCF-DA and stimulated with anti-LFA-1 antibody. Increase of production of ROSs was measured as increase in fluorescence intensity after 1 hour. These experiments were performed with 4 replicates and were repeated 3 times with similar results. Results showing significance difference in fluorescence intensity in comparison with baseline levels are marked with ** for P < .005 or * for P < .05.
