![Figure 1. Effect of patupilone on patient MM cells and peripheral blood mononuclear cells. (A) Patient cells treated for 24 hours in the presence of 10 nM patupilone were compared with untreated controls under a light microscope. MM.1S cells in RPMI media were examined under an Olympus BX60 inverted microscope (Olympus, Lake Success, NY) equipped with Hoffman objective lenses (X10/X20/X40; Diagnostic Instruments, Sterling Heights, MI) connected to a SPOT One Digital Camera (Diagnostic Instruments). Images were then processed using Adobe Photoshop software (Adobe, San Jose, CA) (B, left) Proliferation of tumor cells from 3 different patients with MM was measured by 3H-[dT] uptake 48 hours after initial exposure to IL-6 and patupilone. (Right) Patupilone inhibits growth of patient MM cells adherent to BMSCs. MM patient BMSCs were cultured with or without patient MM cells and in the presence of 0, 1, or 10 nM patupilone. Proliferation was measured at 48 hours. (C) Peripheral blood mononuclear cells (PBMCs; lower panel) from healthy donors either unstimulated (▴) or stimulated with PHA (▪), were treated with patupilone. Survival was determined using MTS assay. Error bars indicate ± standard deviation of triplicate experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/1/10.1182_blood-2004-06-2499/6/zh80010571480001.jpeg?Expires=1735593699&Signature=B0dVVAmzhEyYaqWARaaFYp9hYwtYtNJDgQytt~iofHxaVZ0RwFfwTUnJ60PhdAuDSgvAeCeiBd6b5O1wbpFlHWIwtaLhfvadD~e6jnqgW1LYo6eNxxLifyOmdmhNBsO9SPgObgJ8BX5jsvElEVtoiT1bFr8R0zK3ndDO-pOIuqlFMPMs2icJDrDuAqQcnzze6OzdlV6G-xPFAmSAMrTnpD7aeWtIN6n5eZTRvbFBgx~b2pb4xbukXxpIyRwflUfAIvA6r0eFSQGDX~FcIF5ISPbiKsDhsU7qHDnpkRuK3cYZv~W4qFcVYNTlei3OqGPgPgWtgi-gf5cz4TUW-G-Sng__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of patupilone on patient MM cells and peripheral blood mononuclear cells. (A) Patient cells treated for 24 hours in the presence of 10 nM patupilone were compared with untreated controls under a light microscope. MM.1S cells in RPMI media were examined under an Olympus BX60 inverted microscope (Olympus, Lake Success, NY) equipped with Hoffman objective lenses (X10/X20/X40; Diagnostic Instruments, Sterling Heights, MI) connected to a SPOT One Digital Camera (Diagnostic Instruments). Images were then processed using Adobe Photoshop software (Adobe, San Jose, CA) (B, left) Proliferation of tumor cells from 3 different patients with MM was measured by 3H-[dT] uptake 48 hours after initial exposure to IL-6 and patupilone. (Right) Patupilone inhibits growth of patient MM cells adherent to BMSCs. MM patient BMSCs were cultured with or without patient MM cells and in the presence of 0, 1, or 10 nM patupilone. Proliferation was measured at 48 hours. (C) Peripheral blood mononuclear cells (PBMCs; lower panel) from healthy donors either unstimulated (▴) or stimulated with PHA (▪), were treated with patupilone. Survival was determined using MTS assay. Error bars indicate ± standard deviation of triplicate experiments.
