Figure 1.
Lymphocyte homing in C2GlcNAcT-I-/- lymphoid organs. Naive donor lymphocytes from pooled MLNs and PLNs of T-GFP mice were TRITC labeled and injected into recipient mice. Lymphoid organs were harvested after 1 hour (A) and 24 hours (B), and the ratio of total homed T cells to B cells was measured by flow cytometry. The dashed line indicates a homing ratio of 1; that is, homing of both subsets was equivalent. Total numbers of homed T cells (C-D) and B cells (E-F) were measured after 1 hour (C, E) and 24 hours (D, F). Homed cell numbers were normalized to the number of injected cells. *P < .05 and **P < .01 versus WT. n = 5 to 7 mice per group. Error bars indicate SEM.