Figure 2.
Intravital microscopy of WT and C2GlcNAcT-I-/- PLN HEVs. The rolling fraction of T cells (A) and B cells (C) was determined as the percentage of rolling cells in the total flux of cells in each HEV. Sticking efficiency of T cells (B) and B cells (D) was calculated as the percentage of adherent cells (30 seconds or longer) in the total lymphocyte flux through each HEV. *P < .05 and **P < .01 versus WT. For WT, n = 4 mice. For T cells, order I, n = 2 venules; order II, 5; order III, 6; order IV, 5; order V, 5. For B cells, order I, n = 5 venules; order II, 8; order III, 12; order IV, 9; order V, 9. C2GlcNAcT-I-/-, n = 4 mice. For T cells, order I, n = 3 venules; order II, 6; order III, 9; order IV, 10; order V, 9. For B cells, order I, 5 venules; order II, 5; order III, 10; order IV, 13; order V, 8 venules.