American Society of Hematology

Figure 7.

$Figure 7. PML I inhibits the proliferation and stimulates the differentiation of murine myeloid progenitor cells in response to G-CSF. (A) Expression of PML protein in L-G infectants. Cell lysates were prepared in SDS-PAGE sample buffer and then analyzed by Western blot using anti-HA antibody. Subfraction of PML protein modified by SUMO-1 is indicated. (B) The L-G infectants were cultured in the presence of 5 ng/mL G-CSF. The relative number of viable cells is shown. (C,D) Morphology of L-G infectants exposed to G-CSF for 6 days (C) and 8 days (D) were tested by staining with May-Gruenwald and Giemsa solutions. (E,F) Percentage of L-G infectants differentiated by G-CSF at day 6 (E) and day 8 (F). Immature includes myeloblasts and promyelocytes; intermediate, myelocytes and metamyelocytes; and mature, band cells and segmented neutrophil cells.$

PML I inhibits the proliferation and stimulates the differentiation of murine myeloid progenitor cells in response to G-CSF. (A) Expression of PML protein in L-G infectants. Cell lysates were prepared in SDS-PAGE sample buffer and then analyzed by Western blot using anti-HA antibody. Subfraction of PML protein modified by SUMO-1 is indicated. (B) The L-G infectants were cultured in the presence of 5 ng/mL G-CSF. The relative number of viable cells is shown. (C,D) Morphology of L-G infectants exposed to G-CSF for 6 days (C) and 8 days (D) were tested by staining with May-Gruenwald and Giemsa solutions. (E,F) Percentage of L-G infectants differentiated by G-CSF at day 6 (E) and day 8 (F). Immature includes myeloblasts and promyelocytes; intermediate, myelocytes and metamyelocytes; and mature, band cells and segmented neutrophil cells.

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