Improved differentiation of human erythroblasts in the presence of T3.Human erythroblasts were induced to differentiate either in the presence or absence of T3 (1 μM). (A) Cells were analyzed by cytocentrifugation plus histochemical staining (Figure 6). Enucleated cells observed at day 6 of differentiation in the presence of T3 are indicated by arrows. Image acquisition was performed as described for Figure 1. Proliferation rates (B) and volume decrease (C) during differentiation were determined daily (Figure 6) using an electronic cell counter. (D) Quantitation of hemoglobin accumulation (see “Materials and methods”; 500 AU corresponds to 11.0 pg hemoglobin). Error bars in panels B-D represent the SD of mean, n = 5. (E) Changes in cell size distribution during differentiation plus T3 (compare with Figure 6D).
