Effects of redox status on permeability. (A) Single PSAC recording on an infected RBC in solution A with 1 mM DTE in the bath and pipette. Seal resistance equal to 230 GΩ; V_m equal to -100 mV; scale bar 2 pA/100 ms. Additional analyses revealed unchanged single-channel chord conductance, voltage dependence, and open/closed dwell-time distributions (not shown). (B) Osmotic lysis of infected cells in sorbitol lysis solution with (red curve) or without (black) 1 mM DTE. Increasing the DTE concentration to 50 mM or adding preincubation with DTE also had no effect; similar results were obtained with the 8 parasite isolates listed in “Results” and in ¹⁴C-sorbitol uptake studies (not shown). (C) Osmotic lysis of uninfected RBCs in sorbitol lysis solution after preincubation with (red curve) or without (black curve) 1 mM t-butyl hydroperoxide for 15 minutes with a 2.5-hour postincubation period at 37°C, as in Huber et al.¹⁰  Increasing the postincubation period to 24 hours also had no effect (not shown). Although the transmittance values are presented in arbitrary units, their scaling is conserved in panels B and C so that %T equal to 80 corresponds to complete osmotic lysis.