Cytofluorimetric analysis of immunomagnetically purified CD34⁺ cells. Immunomagnetically purified CD34⁺ cells from PBMCs of one representative patient with acute myocardial infarction (AMI) at admission were stained with FITC–anti-CD34, PE-IgG1, and PerCp-streptavidin–biotinylated IgG1 (A), with FITC-CD34, PE-CD133, and PerCp-streptavidin–biotinylated anti–VEGFR-2 (B), or with FITC-CD34, PE-CD117, and PerCp-streptavidin–biotinylated anti–VEGFR-2 (C). The same staining procedure was performed on purified CD34⁺ cells from one representative healthy control (CTRL; D-F). Results are expressed as percentage of CD34⁺ cells.