Follow-up study and phenotype of endothelial colonies from patients with AMI. Number of endothelial colonies obtained from PBMCs of healthy controls (CTRLs) or patients with AMI tested at T0, 24 hours, (24h), and 7 days (7d) after onset of AMI (A); the lines represent the median values. Panels B and C show an endothelial colony coexpressing both VE-cadherin (B; green) and CD31 (C; red) antigens. Specimens were stained by indirect immunolabeling using secondary antibodies conjugated with AlexaFluor-988 (green) or AlexaFluor-594 (red) fluorochromes (Molecular Probes, Eugene, OR). Mowiol4-88 (Calbiochem, La Jolla, CA) was used for mounting medium. Micrographs were captured using an Olympus IX71 inverted microscope (Olympus Europa GmbH, Hamburg, Germany) equipped with a CPlan F1 10×/0.30 objective, an Olympia Camedia C-3030 zoom with a 3× optical plus 2.5× digital zoom (Olympus), and Camedia Master 2.0 software (Olympus). Original magnification, × 250.