Figure 4.
Deletion of TNF receptors sensitizes macrophages to RANKL-induced activation of ERK1/2. (A) Time- and dose-dependent ERK1/2 activation by RANKL in wild-type and TNF receptor-deleted macrophages. 1.0 × 106 cells were treated with 5 nM RANKL for the indicated times or with the indicated concentrations of RANKL for 15 minutes. Whole-cell extract (50 μg) was resolved by 10% SDS-PAGE and electrotransferred to a nitrocellulose membrane. Western blot analysis was performed using phospho-specific anti-ERK1/2 antibody as described in “Materials and methods.” The same membrane was reblotted with anti-ERK2 antibody. (B) Graphical representation of the results shown in panel A.
