Figure 5.
Deletion of TNF receptors sensitizes macrophages to RANKL-induced activation of p38 MAPK. (A) Time- and dose-dependent p38 MAPK activation by RANKL in wild-type and TNF receptor-deleted macrophages. 1.0 × 106 cells were treated with 5 nM RANKL for the indicated times or with the indicated concentrations of RANKL for 15 minutes. Whole-cell extract (50 μg) was resolved by 10% SDS-PAGE and electrotransferred to a nitrocellulose membrane. Western blot analysis was performed using phospho-specific anti-p38 MAPK antibody as described in “Materials and methods.” The same membrane was reblotted with anti-p38 MAPK antibody. (B) Graphical representation of the results shown in panel A.
