CD150 is linked to the ERK1/2 and Akt pathways in HD cell lines. (A) SH2D1A coprecipitated with CD150 in the L1236 cell line but did not associate with CD150 in the KM-H2 and L428 HD cell lines (bottom panel). Anti-SH2D1A Western blot on CD150 immunoprecipitates. SHIP and SHP-2 did not associate with CD150 in HD cell lines (top and middle panels). Western blot with anti-SHP-2 and anti-SHIP. One of 3 experiments. (B) All CD150 splice forms (mCD150, sCD150, and tCD150) and SH2D1A were expressed in HD cell lines. RT-PCR analysis. MP-1 lymphoblastoid cell line, BJAB, and DG75 Burkitt lymphoma cell lines served as positive and negative controls. (C) Cross-linking of CD150 on HD cell lines affects the level of ERK1/2 and Akt phosphorylation. Anti-pERK1/2 and anti-pAkt Western blot analyses on cell lysates. Western blot with antiactin controlled for equal loading (bottom panels).