TfR2 increases in HuH7 cells, but not in K562 or TRVb2 cells. Cells were cultured for 24 hours after the addition of 25 μM diferric Tf to the medium. The level of TfR2 in lysates from HuH7 (50 μg), K562 (20 μg), and TRVb2 (10 μg) cells was determined by Western blot with chemiluminescence detection. In cells endogenously expressing TfR2, treatment with diferric Tf increased TfR2 in HuH7 human hepatoma cells but not in K562 erythroleukemia cells. TRVb cells stably transfected with TfR2 (TRVb2) did not respond to diferric Tf.