American Society of Hematology

Figure 6.

$Figure 6. Tissue factor is associated with Brij 58 detergent-resistant, cholesterol-based membrane domains. (A). WI-38 cells were lysed in 0.5% of the indicated detergent for 30 minutes at 4°C, and the postnuclear supernatants were fractionated into pellets (P) and supernatants (S) by centrifugation at 16 000g for 30 minutes and analyzed by immunoblotting. (B). WI-38 cells were cholesterol-depleted with mβCD (10 mM for 30 minutes) at 37°C and subsequently lysed in Brij 58. The samples were analyzed for TF by Western blotting, and the signals were quantitated by densitometry (n = 5, mean ± SE).$

Tissue factor is associated with Brij 58 detergent-resistant, cholesterol-based membrane domains. (A). WI-38 cells were lysed in 0.5% of the indicated detergent for 30 minutes at 4°C, and the postnuclear supernatants were fractionated into pellets (P) and supernatants (S) by centrifugation at 16 000g for 30 minutes and analyzed by immunoblotting. (B). WI-38 cells were cholesterol-depleted with mβCD (10 mM for 30 minutes) at 37°C and subsequently lysed in Brij 58. The samples were analyzed for TF by Western blotting, and the signals were quantitated by densitometry (n = 5, mean ± SE).

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