Time-dependent shifts in the volume distribution and in MCV and MCHC of AA RBCs during Ca²⁺-induced dehydration in the presence and absence of sodium orthovanadate. Experimental details are given in the Table 1 footnote. (A) Arrows indicate the time direction of displacement of the curves representing the RBC V distributions during Ca²⁺-induced dehydration in the presence (ii) and absence (i) of 1 mM sodium orthovanadate. Time intervals from the initial curve are those indicated in the first column of Table 1. The area under each of the curves was similar, as confirmed by the nearly identical number of cells counted in the Advia for each sample (± 0.6%). Therefore, the gradual increase in the height of the distribution curves toward the completion of dehydration reflects a narrowing of the volume distribution (declining SD of MCV, as shown in Table 1 and in the insets in panel B). (B) MCVs (in femtoliters; •) and MCHCs (in g/dL; ○) are plotted as a function of time after A23187 addition to illustrate the inverse MCV-MCHC changes during Gardos-channel-mediated dehydration in the presence (ii) and absence (i) of 1 mM sodium orthovanadate. Insets show changes in SDs of the V distributions with time after A23187 addition.