Figure 2.
Retinoid-dependent expression of CHOP mRNA in acute leukemia cell lines. NB4, NB4-R2, HL60, and HL60R cells were incubated with ATRA (5 × 10-7 M) for various durations and were harvested for total RNA. Northern blot analyses were performed using total RNA (10-30 μg/lane) and hybridized with 32P-labeled full-length C/EBPα, C/EBPϵ, or CHOP cDNA probes. Blots were stripped and rehybridized with β-actin, GAPDH, or 28S probes to confirm equal loading of the samples. (A) Time-dependent C/EBPα, C/EBPϵ, and CHOP mRNA expression in NB4 cells. (B) Time-dependent CHOP mRNA expression in HL60 cells and retinoid-resistant HL60R and NB4-R2 cells.
