Septic plasma and soluble MD-2, but not normal plasma, support FITC-LPS (1 μg/mL) binding to TLR4-HEK293 cells. Symbols represent septic plasma (○), soluble MD-2 (♦), and normal plasma (□). Mean ± 1 SD of triplicates. This is 1 representative experiment of 2 experiments. Similar results were obtained with 200 ng/mL FITC-LPS.