Antiangiogenic effects of CCI-779. (A) Mice challenged and treated as in Figure 3, and angiogenesis assayed as described in “Materials and methods.” Results represent CD34⁺ area of microscopic field (original magnification, ×20) stained positively, assessed as described in “Materials and methods.” Data are mean ± SD, n = 10 fields, for each group. Asterisks denote significant differences (P < .05) between control and CCI-779-treated mice. (B) Representative slides of CD34-stained sections for 8226- and OPM-2-challenged mice treated with vehicle or 20 mg/kg CCI-779 at day 6. Original magnification, ×40. Arrows show several microvessels.