American Society of Hematology

Figure 5.

$Figure 5. Effects of decreasing metabolic activity (4°C) on mobilization of vesicular 59Fe. Chelators (1 mM DP or 0.1 mM SIH) were included either during periods 4 and 5 (▵) or dissolved in the H2O used to lyse 59Fe-reticulocytes (•). Following incubations of 59Fe-reticulocytes with the chelators, radioactivity was measured in the media, and that value was added to that of the EtOH soluble fraction. This sum represents the total chelator-bound 59Fe. The data are expressed as the percentage of the total cellular radioactivity as it was after the membrane-associated radioiron was displaced by 56Fe-Tf (Figure 1, end of period 4). As a control, the EtOH soluble 59Fe was extracted from untreated, 59Fe-labeled cells (□).$

Effects of decreasing metabolic activity (4°C) on mobilization of vesicular ⁵⁹Fe. Chelators (1 mM DP or 0.1 mM SIH) were included either during periods 4 and 5 (▵) or dissolved in the H₂O used to lyse ⁵⁹Fe-reticulocytes (•). Following incubations of ⁵⁹Fe-reticulocytes with the chelators, radioactivity was measured in the media, and that value was added to that of the EtOH soluble fraction. This sum represents the total chelator-bound ⁵⁹Fe. The data are expressed as the percentage of the total cellular radioactivity as it was after the membrane-associated radioiron was displaced by ⁵⁶Fe-Tf (Figure 1, end of period 4). As a control, the EtOH soluble ⁵⁹Fe was extracted from untreated, ⁵⁹Fe-labeled cells (□).

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