Effects of wortmannin (WT) and N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide (W-7) and their combination on the use of endosomal ⁵⁹Fe for heme synthesis. WT (A) and W-7 (B) were included during the last 30 minutes of period 4 and all of period 5 (Figure 1); the data are displayed as in the previous 3 figures. (C) The results of incubation WT or W-7 (as in panels A and B) or the combination of WT and W-7 are presented as the percentage of increase in ⁵⁹Fe-heme content from time = 0 (ie, at the beginning of period 5).