Induction of proliferation of autologous lymphocytes on presentation of a soluble antigen, FLU. The lack of T-stimulatory activity of IL-16/TPO-DCs was confirmed when the proliferation of autologous T lymphocytes on presentation of FLU was tested. The capacity of DCs obtained in the different cytokine combinations to induce T-cell proliferation was compared. Proliferating lymphocytes were identified in density plots as CD4⁺/BrdU⁺ double-positive cells. Control cultures included monocyte-depleted PBMCs alone (left column), cocultured with unpulsed DCs (middle column), or antigen-specific proliferation assessed in cultures with FLU-pulsed DCs (right column). The numbers represent the percentage of cells in each quadrant. One representative of 3 independent experiments is shown.