Effects of K-11706 and K-13144 on the inhibition of Epo protein production from Hep3B cells by IL-1β and TNF-α. Aliquots of 3 × 10⁶ Hep3B cells were incubated with 15 U/mL rhIL-1β, 220 U/mL rhTNF-α or 10 nM, 50 nM, or 100 nM K-11706 or 100 nM K-13144 under hypoxic conditions (1% O₂) for 24 hours. Epo protein was measured by ELISA. Eight separate experiments were performed. Error bars represent 1 SD. ^* indicates significance compared with control, P < .005; ^**, significance compared with 15 U/mL IL-1β, P < .025; ^***, significance compared with 15 U/mL IL-1β, P < .005; ^****, significance compared with 220 U/mL TNF-α, P < .005.