Western blot analysis of neutrophil membranes from the patient and 4 of her daughters. Neutrophil membranes were solubilized and subjected to electrophoresis, blotted, and probed with mAbs against gp91phox (A) or against p22phox (B) as indicated in “Methods.” Lane 1, control neutrophil from a patient with X-CGD; lane 2, control neutrophils from a healthy individual; lane 3, neutrophils from the patient (no. 9); lane 4, neutrophils from daughter no. 20; lane 5, neutrophils from daughter no. 16; lane 6, neutrophils from daughter no. 17; and lane 7, neutrophils from daughter no. 15.