Figure 2.
Dose-dependent effect of LPA supplementation on cell tryptase expression. SDS-PAGE immunoblot (top) showing immunoreactivity for tryptase with an antibody that recognized α- and β-human tryptases in whole cell lysates prepared from 1 × 105 cells developed for 3 weeks in serum-free medium of SCF, IL-6, and IL-10, with or without the indicated concentrations of LPA. Data are from 1 of 3 representative experiments. Percentages of cells exhibiting tryptase immunoreactivity (bottom) with the same antibody are shown as the mean ± SEM from 3 experiments performed. *P < .05.
