Figure 1.
![Figure 1. Effects of CC-5013, rapamycin, and their combination on proliferation of MM cell lines and PBMCs. (A) Time- and dose-dependent inhibition of proliferation in sensitive MM cell lines (MM1.S, OPM2, SB645), demonstrated by measuring thymidine [3H-TdR] uptake during the last 8 hours of 48-hour cultures, in the presence of CC-5013 (μM), rapamycin (nM), or both in fixed-dose treatment. (B) Time- and dose-dependent inhibition of proliferation demonstrated by MTT in MM1.S cells. (C) Inhibition of DNA synthesis as measured by 3H-TdR uptake in resistant MM cell lines treated with CC-5013 and rapamycin. (D) Lack of toxicity of combined therapy on PBMCs, demonstrated by MTT assay. Error bars indicate one standard deviation.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/104/13/10.1182_blood-2004-06-2281/6/zh80240470780001.jpeg?Expires=1735537754&Signature=Nwiw3gw6JO5F0GtxbEuUWr1M-sFWZjzFc6ALwJr6kE5jTsPxPbE~jRKfBRelOaX9eW~PyB6jqrWljoH5tttL4MgT7-R64or46iTT055OymRczBevInutS~goTfSYyiHzzPYdE5YRzpC5KXLTp6R-mQzPPU7KgTUw44ACVmLo6Ok34VtnneLEQn036mOZ6gwQnucQQ5G9WQIxgibKiFBMBCp~iylf1FOddkmCcdiLJpFMXY10h9CxS12wB51p1QPKwrekZV9cU4-iYWCCZW1pA-AylLDnRxpaKKc1TBwxPUJouBhcUnuz5M5ov4esFqQT2Tw70QGxw7PsK6Y-ddxl3Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effects of CC-5013, rapamycin, and their combination on proliferation of MM cell lines and PBMCs. (A) Time- and dose-dependent inhibition of proliferation in sensitive MM cell lines (MM1.S, OPM2, SB645), demonstrated by measuring thymidine [3H-TdR] uptake during the last 8 hours of 48-hour cultures, in the presence of CC-5013 (μM), rapamycin (nM), or both in fixed-dose treatment. (B) Time- and dose-dependent inhibition of proliferation demonstrated by MTT in MM1.S cells. (C) Inhibition of DNA synthesis as measured by 3H-TdR uptake in resistant MM cell lines treated with CC-5013 and rapamycin. (D) Lack of toxicity of combined therapy on PBMCs, demonstrated by MTT assay. Error bars indicate one standard deviation.
