Generation and analyses of TG mice. (A) Construct used for embryo injection. (B) Southern blot from tails of generated mice. Bands (human CD2) indicate the construct integrated in the DNA of TG mice (TG). Bands are absent in wild-type mice (WT). (C) mRNA evaluated by ribonuclease protection assay (RPA). GILZ mRNA levels were analyzed in thymocytes from WT (lane 3) or TG (lane 4) mice. GILZ or β-actin probes are shown in lanes 1 and 2, respectively. (D) Quantitative analysis of RPA experiment shown in panel C. (E) Western blot of GILZ protein levels in thymocytes from WT mice left untreated (control, C; lane 1) or treated with 0.01, 0.1, 1, or 10 mg/kg DEX (lanes 2, 3, 4, and 5, respectively) or in thymocytes from 3 TG mice (TG no. 1, TG no. 2, TG no. 3).