Thymocyte Bcl-xL expression. (A) RPA analysis shows mRNA levels of Bcl-xL, Bak, and Bax from a representative experiment. Bars represent quantitative analysis of Bcl-xL (Bcl-xL/L32 mRNA ratio), Bax (Bax/L32 mRNA ratio), or Bak (Bak/L32 mRNA ratio) mRNA levels in WT and TG thymocytes from 3 independent experiments. (B) Western blot analysis of Bcl-xL (labeled “Bcl-xL”) and β-tubulin (“β-tub”) proteins and, on the right, the quantitative analysis (bcl-xL/β-tubulin ratio) of WT and TG thymocytes from 3 independent experiments. Bars represent the mean values ± 1 SEM. ^*P < .05.