Effect of GST-TAT-GILZ fusion protein on WT thymocytes. (A) Western blot analysis of thymocyte protein untreated or treated for 30 minutes, 1 hour, or 2 hours with either GST-TAT (1) or with GST-TAT-GILZ (2), revealed with the anti-GST mAb. (B)Western blot of Bcl-xL expression on WT thymocytes untreated or treated for different times (30 minutes, 1 hour, or 2 hours) with either GST-TAT-GILZ (GILZ) or GST-TAT (TAT). (C) Bars indicate apoptosis of WT thymocytes (left) or splenocytes (right) after 24 hours of treatment with either GST-TAT (TAT; □) or GILZ-GST-TAT (GILZ; ▦). Bars represent the mean values ± 1 SEM from 3 independent experiments. ^*P < .05.