Histologic examination of the liver. Four-month-old wild-type and homozygous TfR2 Y245X mutant female sibling mice were injected with either PBS or 5 mg iron-dextran intraperitoneally twice a week for 3 weeks. One week after the last injection, the livers were subjected to histologic analysis. (A, E) The liver of a PBS-treated wild-type mouse. (B, F) The liver of a PBS-treated homozygous TfR2 Y245X mutant mouse. (C, G) The liver of an iron-loaded wild-type mouse. (D, H) The liver of an iron-loaded homozygous TfR2 Y245X mutant mouse. (A-D) Hematoxylin eosin staining. (E-H) Perl Prussian blue staining for the detection of iron storage. Original objective magnification of these panels was × 20.