Recombinant megaCD40L induces IL-12p70 and TNF production in patients with SzS. (A) Dendritic cells derived from CD34+ stem cells of healthy donors were stimulated with the indicated reagents. MegaCD40L (ACRP30-CD40L) and ACRP30 were used at 100 ng/mL. After 18 hours, CD83 surface expression was monitored by immunostaining and flow cytometry analysis. Fluorescence histograms are shown. (B-C) Total PBMCs from a healthy donor and 2 patients with SzS were stimulated with the indicated reagents. IFN-γ was used at 500 IU/mL, LPS at 1 μg/mL, and megaCD40L at 100 ng/mL. After 24 hours, supernatants were harvested and assayed for IL-12p70 (B) and TNF-α (C) production.