Organ infiltration by myeloid cells after induction of BCR-ABL expression in double-transgenic animals. (A) Peripheral blood smear showing leukocytosis and neutrophilia in a diseased double-transgenic mouse. (B) Histology of the spleen demonstrating extramedullary myeloid hematopoiesis (arrows pointing to myeloid cells). Inset: Myeloid cells in the cytospin of the spleen from the same animal. (C) Histology of the liver. Perivascular infiltration of the liver by hematopoietic cells (arrows point to myeloid cells). Inset: Part of the same organ at higher magnification. (D) Histology of the small intestine showing infiltration of the lamina propria by myeloid cells (arrow, and magnified in the inset). (E) Histology of a kidney from mouse W207 showing disruption of the kidney architecture and massive invasion by hematopoietic cells. Inset: Cells at higher magnification. (F) Histology of the kidney of animal W194 demonstrating parenchymal tissue necrosis with some invading myeloid cells still present (arrow with solid line); note the presence of crystals in the lower part of the picture (arrow with broken line). (G) Fluid recovered from the perinephric cyst of the same animal containing high numbers of neutrophils. (H) Cytospin of the bone marrow showing predominance of myelopoiesis and suppression of the erythroid and lymphoid compartment in the bone marrow. Original magnification, × 60 (B-C) or × 100 (A;D, inset). Original magnifications, × 25 (D), × 60 (A-C, E-F), and × 100 (G-H, insets). Stains were Wright Giemsa for panels A, G, and H, and hematoxylin and eosin for panels B-F.