BCR-ABL RNA and protein are expressed in hematopoietic tissues of diseased mice. (A) Northern blot analysis of RNA isolated from organs, as indicated above the blot from 5 different SCLtTA/BCR-ABL mice (W107, W109, W118, W110, W114, as described in Table 1). RNA from the bone marrow of a SCLtTA single-transgenic (-) mouse and from the affected meninges of an MMTV tTA-BCR-ABL⁸  double-transgenic (+) mouse served as negative and positive controls, respectively. The lower panel shows 18S and 28S RNA as loading controls. (B) Western blot showing BCR-ABL and c-abl protein expression in the spleens of 4 SCLtTA/BCR-ABL and 5 control mice. SB, B, and S designate SCLtTA/BCR-ABL double-transgenic (dTG), BCR-ABL single-transgenic, and SCLtTA single-transgenic mice, respectively. (C) Western blot of cells from the small intestine (sm.intest.), lung, and bone marrow (bm) of SCLtTA/BCR-ABL double-transgenic (SB), BCR-ABL single-transgenic (B), SCLtTA single-transgenic (S), and wild-type (wt) mice.