Effect of pentoxifylline (PTF), jasplakinolide (JK), and monensin on BLyS secretion. Twenty-hour G-CSF–treated neutrophils were incubated for 1 hour with or without PTF, JK, or monensin before TNF-α or fMLP addition. After 3 hours, cell-free supernatants were harvested for ELISA determination of antigenic BLyS, lactoferrin, MMP-9, and albumin. Data report the mean ± SEM of the percentages of inhibition on TNF-α/fMLP–induced release of BLyS, lactoferrin, MMP-9, and albumin provoked by PTF, JK, and monensin over G-CSF–treated cells (calculated from 4 experiments).