Kinetic analysis of hematopoiesis-associated gene expression by QRT-PCR. Human-specific primers listed in Table 1 were used to amplify indicated genes during H1 and H9 differentiation in OP9 coculture. Pictures under each graph show corresponding agarose gel electrophoresis of QPCR from H1 cell samples. Gene expression relative to bone marrow RNA was calculated using ΔΔCt method as described in “Materials and methods.”